Ultra-sensitive detection of E. coli cells for chemical sensors and biosensors.
One of the most dangerous food borne and water borne pathogens is E. coli 0157:H7. It is highly virulent and is infectious with a very low dose such that 10 to 100 CFU can lead to infection. methods for monitoring this pathogen in food production and water include a culturing and colony counting method, polymerase chain reaction, and immunological methods. These methods have low sensitivity, less specificity, and are time-consuming.
The inventors have utilized self-assembly of thermally-reduced monolayer graphene oxide (TRMGO) nanosheets on photolithographically patterned gold electrodes for highly sensitive detection of E. coli 0157:H7. This fabrication method by a solution process is suitable for mass-production of GO field effect transistor (FET) sensors. The TRMGO FET device shows great electronic stability and high sensitivity to E. coli cells with a concentration as low as 10 CFU per milliliter. The inventors have shown that this immunosensor has high sensitivity with a short response time as well as high reproducibility.
The UWM Research Foundation has licensed the above patents to NanoAffix Science, LLC (http://www.nanoaffix.com/) and together the parties are working find additional partners to develop the technology into a final product.
Junhong Chen PhD
For further information please contact: Smruti Patil, Ph.D., IPMM | Licensing Associate | Tel: 414-906-4657