Snapshot Tomography for Tissue and Cell Imaging

OTT#1494

Applications

Pharmaceuticals, Agriculture, Food Processing, Chemical and Petrochemicals.

Target Problems

• Hyperspectral chemical imaging (HCI) is widely used for quality inspection of various active compounds and specimen samples but is incapable of measuring depth information or examine a specimen with a complicated internal structure.
• HCI records only 2D absorption maps, cannot detect the impurities buried inside a 3D volume.

Features/Benefits

• Real-Time Imaging -Real time snapshot of individual cells can be obtained
• Minimally Invasive and Sensitive -The restorative algorithm aids with identification of imaging artifacts
• Quality Assurance -Able to detect impurities, leachates, contaminants, and foreign materials
• Spectral Range –3-D Images and spectra can be easily captured under UV, visible and infrared light

Technology

Inventors at University of Wisconsin, Milwaukee (UWM) have developed snapshot optical tomography technique which can record the 3D tomogram of a micrometer to millimeter sized specimen in a single snapshot. In particular, snapshot holographic optical tomography (SHOT) can instantaneously record the 3D map of complex-valued refractive index (i.e., absorption and refractive index). Furthermore, snapshot projection optical tomography (SPOT) can also instantaneously record the 3D luminescence (e.g., fluorescence, bioluminescence, photoluminescence) when needed.

Chemical identification, including the 3D distribution of active pharmaceutical ingredients, excipient or lyophilizate distributions of tablets can be achieved by combining the tomograms with analysis techniques like FT-IR, Raman, and UV-visible spectroscopy. This technology can provide an opportunity for safe and accurate testing of 100% of tablet or capsule beads.

Intellectual Property

U.S Utility Patent 10,845,759 B2

This technology is available for developmental research support/licensing under either exclusive or non-exclusive terms.

Inventor (s)

Lead Inventor: Yongjin Sung, Ph.D.Assoc.
Professor in Biomedical Engineering and Mechanical EngineeringUniversity of Wisconsin-Milwaukee
Sung Lab Website